Benutzerspezifische Werkzeuge

Novel approaches using 3-dimensional reconstructs

Despite remarkable efforts, metastatic melanoma still presents with significant mortality. In order to improve the increasing requests on new therapeutic alternatives, reliable high throughput screening models are demanded that translate the findings from basic cellular research into clinical applications.

By developing an organotypic full skin equivalent, harboring melanoma tumor spheroids of defined sizes we have invented a cell-based model that recapitulates both the 3-d organization and multicellular complexity of an organ/tumor in vivo but at the same time accommodates systematic experimental intervention. By using this organotypic tumor model we are aiming to elucidate the impact of intra-tumoral diversity and of the surrounding host tissue on the therapeutic success. Above this, the model serves the needs of a fast and reliable tool for pre-clinical analysis of tailored combination therapies to establish individualized melanoma therapy.



Relevant own publications

  1. Montinaro A, Zubiaur IA, Saggau J, Kretz A-L, Ferreira RMM, Hassan O, Kitzig E, Mueller I, El-Bahrawy M, von Karstedt S, Kulms D, Liccardi G, Lemke J, Walczak H: Potent pro-apoptotic combination therapy is highly effective in a broad range of cancers. Cell Death Differ, in press (2021)

  2. Margue C, Philippidou D, Kozar I, Cesi G, Felten P, Kulms D, Letellier E, Haan C, Kreis S: Kinase inhibitor library screening identifies synergistic drug combinations effective in sensitive and resistant melanoma cells. J Exp Clin Cancer Res, 38:56. doi: 10.1186/s13046-019-1038-x (2019);
  3. Müller I, Kulms D: 3D organotypic melanoma-spheroid-skin-model. J Vis Exp, (135). doi: 10.3791/57500 (2018)
  4. Vörsmann H, Gröber F, Walles H, Beissert S, Walczak H, Kulms D: Development of a human three-dimensional organotypic skin-melanoma spheroid model for in vitro drug testing. Cell Death Dis doi: 10.1038/cddis.2013.249. (2013)
  5. Kulms D, Meier F: In vitro models of melanoma. In: Skin Tissue Models, Eds.: AP Marques, RP Pirraco, MT Cerqueria, RL Reis; Elsevier Inc, Camebridge; ISBN: 978-0-12-810545-0 (2018)