Benutzerspezifische Werkzeuge

Experimentelle Hämatologie


PD Dr. med. Sebastian BrennerOA PD Dr. med. Sebastian Brenner

Wissenschaftliche Mitarbeiter

Dr. rer. nat. Sebastian Thieme
Dr. rer. nat. Magdalena Laugsch
Dr. rer. nat. Cornelia Richter
Dr. med. Tobias Gyarfas
Joanna Bandola MSc
David Brandt
Cand. med. Paula Jobst
Katrin Navratiel (MTA)
Jenny Marzahn (MTA)

Betreuung wissenschaftlicher Mitarbeiter in Kooperation

Dr. med. Martin Wermke (Kooperation mit Prof. Buchholz und Prof. Bornhäuser)
Aylin Camgoz, MSc (Kooperation mit Prof. Buchholz und Prof. Bornhäuser)
Dipl. Biol. Sabine Stopp (Kooperation mit Prof. Bornhäuser)
Dipl. Biol. Regina Duryagina (Kooperation mit Prof. Bornhäuser und Prof. Carsten Werner)


  • Erstellung und Korrektur Patienten-spezifischer iPS Zellen zur Therapie der Septischen Granulomatose (BMBF-Projekt in Kooperation mit Dr. Konstantinos Anastassiadis und Prof. Francis Stewart; Dr. Laugsch)
  • Rolle der H3K27 Demethylase UTX bei der Blutstammzell-Migration (SFB 655, B6; Dr. Thieme)
  • Charakterisierung neuer Mechanismen der Migration hämatopoetischer Stammzellen(MeDDrive 2011/2012, Dr. Gyarfas)
  • Rolle der plasmazytoiden dendritischen Zellen bei Asthma bronchiale (DFG Projekt, Joanna Bandola, MSc)
  • Die Rückkopplung der IL-12p70 Antwort durch Ncf1 (p47phox) und seine Bedeutung in der Tumorprävention (DFG, Dr. Richter)
  • shRNA Screen zur Identifikation neuer, an der Blutstammzell-Migration und Zelladhäsion beteiligter Gene
  • Rolle von MCAM auf mesenchymalen Stammzellen (Sabine Stopp)
  • Etablierung eines deutschlandweiten pädiatrischen Reanimations- und Hypothermieregisters

ausgewählte Publikationen

Ortlepp C, Steudel C, Heiderich C, Koch S, Jacobi A, Ryser M, Brenner S, Bornhäuser M, Brors B, Hofmann W-K, Ehninger G, Thiede C. Autotaxin is expressed in FLT3-ITD positive acute myeloid leukemia and hematopoietic stem cells and promotes cell migration and proliferation. Exp Hem. 2013 in press

Thieme S, Gyárfás T, Richter C, Özhan G, Fu J, Alexopoulou D, Muders MH, Michalk I, Jakob C, Dahl A, Klink B, Bandoła J, Bachmann M, Schröck E, Buchholz F, Stewart AF, Weidinger G, Anastassiadis K, Brenner S: The histone demethylase UTX regulates stem cell migration and hematopoiesis. Blood 2012, in press.

Stopp S, Bornhäuser M, Ugarte F, Wobus M, Kuhn M, Thieme S, Brenner S. Expression of the melanoma cell adhesion molecule in human mesenchymal stromal cells regulates proliferation, differentiation, and maintenance of hematopoietic stem and progenitor cells. Haematol 2012, in press.

Rellensmann G, Masjosthusmann K, Brenner S. Therapeutische Hypothermie bei Neugeborenen und Kindern. Intensivmedizin up2date. 2012 Aug(03):193-209.

Hofmann SR, Möller J.C., Paul D., Rösen-Wolff A., Brenner S., Hedrich C.M. Dynamic CpG-DNA methylation of Il10 and Il19 in CD4+ T lymphocytes and macrophages: Effects on tissue-specific gene expression. Klin Padiatr 2012 Mar;224(2):53-60.Hoffmann F, Gräsner J.-T., Brenner S. Etablierung eines Reanimations- und Hypothermieregisters für Kinder. Anästh Intensivmed 2011;52(11):727-729.

Hoffmann F., Rüdiger M., Nicolai T., Brenner S. Retrospektive Umfrage zur Häufigkeit von Reanimationen und Hypothermie - Behandlungen auf pädiatrischen Intensivstationen in Deutschland. DIVI Journal 2011,2(4):20-24.

Hoffmann F, Gräsner J.-T., Brenner S. Etablierung eines Reanimations- und Hypothermieregisters für Kinder. Anästh Intensivmed 2011;52(11):727-729.

Jacobi A, Thieme S, Lehmann R, Ugarte F, Malech HL, Koch S, Thiede C, Müller K, Bornhäuser M, Ryser M, Brenner S. Impact of CXCR4 inhibition on FLT3-ITD positive human AML blasts. Exp Hematol. 2010;38:180-190.

Ugarte F, Ryser M, Thieme S, Fierro FA, Navratiel K, Bornhäuser M, Brenner S. Notch signaling enhances osteogenic differentiation while inhibiting adipogenesis in primary human bone marrow stromal cells. Exp Hematol. 2009 Jul;37(7):867-875.

Ryser MF, Thieme S, Lehmann R, Bornhäuser M, Brenner S. Serum albumin strongly influences SDF-1 dependent migration. Int J Hematol. 2009 Apr;89(3):269-75.

Thieme S, Ryser M, Gentsch M, Brenner S, Stiehler M, Rölfing J, Gelinsky M, Rösen-Wolff A. Stromal derived factor-1a (SDF-1α) directed chemoattraction of transiently CXCR4 overexpressing mesenchymal stem cells into functionalised three-dimensional biomimetic scaffolds. Tissue Eng. 2009 Dec;15(4):687-96.

Pompe T, Glorius S, Bischoff T, Uhlmann I, Kaufmann M, Brenner S, Werner C. Dissecting the impact of matrix anchorage and elasticity in cell adhesion. Biophys J. 2009 Oct 21;97(8):2154-63.

Fierro FA, Brenner S, Oelschlaegel U, Jacobi A, Knoth H, Ehninger G, Illmer T, Bornhäuser M: Combining SDF-1/CXCR4 antagonism and chemotherapy in relapsed acute myeloid leukemia. Leukemia. 2009 Feb;23(2):393-6.

Moreno-Carranza B, Gentsch M, Stein S, Schambach A, Santilli G, Rudolf E, Ryser MF, Haria S, Thrasher AJ, Baum C, Brenner S, Grez M. Transgene optimization significantly improves SIN vector titers, gp91phox expression and reconstitution of superoxide production in X-CGD cells. Gene Ther. 2009 Jan16(1):111-8.

Ryser MF, Ugarte F, Gentsch M, Lehmann R, Bornhäuser M, Brenner S. S1P1 overexpression stimulates S1P dependent chemotaxis of human hematopoietic stem cells but strongly inhibits SDF-1/CXCR4 dependent migration and in vivo homing. Mol Immunol. 2008 Nov;46,166-71.

Ryser MF, Ugarte F, Thieme S, Gentsch M, Bornhäuser M, Roesen-Wolff A, Brenner S. mRNA transfection of a CXCR4-GFP fusion protein - generated by a simple three step PCR protocol - results in efficient migration of primary mesenchymal stem cells. Tissue Eng Part C Methods. 2008 Sep;14,179-84.

Brenner S, Ryser MF, Whiting-Theobald NL, Gentsch M, Linton GF, Malech HL. The late dividing population of gamma-retroviral vector transduced human mobilized peripheral blood progenitor cells contributes most to gene-marked cell engraftment in nonobese diabetic/severe combined immunodeficient mice. Stem Cells. 2007 Jul;25(7):1807-13.

Ryser MF, Roesler J, Gentsch M, Brenner S. Gene Therapy for Chronic Granulomatous Disease. Expert Opinion on Biological Therapy. 2007 Dec;7(12):1799-1809.

Chronic granulomatous disease (CGD) and complete myeloperoxidase deficiency both yield strongly reduced dihydrorhodamine 123 test signals but can be easily discerned in routine testing for CGD. Mauch L, Lun A, O'Gorman MR, Harris JS, Schulze I, Zychlinsky A, Fuchs T, Oelschlagel U, Brenner S, Kutter D, Rosen-Wolff A, Roesler. J. Clin Chem. 2007 May;53(5):890-6.

Bleesing JJ, Souto-Carneiro MM, Savage WJ, Brown MR, Martinez C, Yavuz S, Brenner S, Siegel RM, Horwitz ME, Lipsky PE, Malech HL, Fleisher TA. Patients with Chronic Granulomatous Disease Have a Reduced Peripheral Blood Memory B Cell Compartment. J Immunol. 2006 Jun 1;176(11):7096-7103.

Brenner S, Ryser MF, Choi U, Whiting-Theobald N, Kuhlisch E, Linton G, Kang E, Lehmann R, Rosen-Wolff A, Rudikoff AG, Farese AM, MacVittie TJ, Roesler J, Horwitz ME, Malech HL. Polyclonal long-term MFGS-gp91phox marking in rhesus macaques after non-myeloablative transplantation with transduced autologous peripheral blood progenitor cells. Mol Ther. 2006 Aug;14(2):202-11.

Roesler J, Koch A, Porksen G, von Bernuth H, Brenner S, Hahn G, Fischer R, Lorenz N, Gahr M, Rosen-Wolff A. Benefit assessment of preventive medical check-ups in patients suffering from chronic granulomatous disease (CGD). J Eval Clin Pract. 2005 Dec;11(6):513-21.

Kang EM, Zickler PP, Burns S, Langemeijer SM, Brenner S, Phang OA, Patterson N, Harlan D, Tisdale JF. Hematopoietic stem cell transplantation prevents diabetes in NOD mice but does not contribute to significant islet cell regeneration once disease is established. Exp Hematol. 2005 Jun;33(6):699-705.

Kawai T, Choi U, Whiting-Theobald NL, Linton GF, Brenner S, Sechler JM, Murphy PM, Malech HL. Enhanced function with decreased internalization of carboxy-terminus truncated CXCR4 responsible for WHIM syndrome. Exp Hematol. 2005 Apr;33(4):460-8.

Ueda T, Brenner S, Malech HL, Langemeijer SM, Perl S, Kirby M, Phang OA, Krouse AE, Donahue RE, Kang EM, Tisdale JF. Cloning and functional analysis of the rhesus macaque ABCG2 gene. Forced expression confers an SP phenotype among hematopoietic stem cell progeny in vivo. J Biol Chem. 2005 Jan 14;280(2):991-8.

Brenner S, Whiting-Theobald N, Kawai T, Linton GF, Rudikoff AG, Choi U, Ryser MF, Murphy PM, Sechler JM, Malech HL. CXCR4-transgene expression significantly improves marrow engraftment of cultured hematopoietic stem cells. Stem Cells. 2004;22(7):1128-33.

Malech HL, Choi U, Brenner S. Progress toward effective gene therapy for chronic granulomatous disease. Jpn J Infect Dis. 2004 Oct;57(5):S27-8.

Brenner S, Whiting-Theobald NL, Linton GF, Holmes KL, Anderson-Cohen M, Kelly PF, Vanin EF, Pilon AM, Bodine DM, Horwitz ME, Malech HL. Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. Blood. 2003 Oct 15;102(8):2789-97.

Anderson-Cohen M, Holland SM, Kuhns DB, Fleisher TA, Ding L, Brenner S, Malech HL, Roesler J. Severe phenotype of chronic granulomatous disease presenting in a female with a de novo mutation in gp91-phox and a non familial, extremely skewed X chromosome inactivation. Clin Immunol. 2003 Dec;109(3):308-17.

Geiszt M, Lekstrom K, Brenner S, Hewitt SM, Dana R, Malech HL, Leto TL. NAD(P)H oxidase 1, a product of differentiated colon epithelial cells, can partially replace glycoprotein 91phox in the regulated production of superoxide by phagocytes. J Immunol. 2003 Jul 1;171(1):299-306.

Brenner S, Malech HL. Current developments in the design of onco-retrovirus and lentivirus vector systems for hematopoietic cell gene therapy. Biochim Biophys Acta. 2003 Apr 7;1640(1):1-24.

Brenner S, Roesler J, Bukovsky AA, Whiting-Theobald N, Dull T, Kelly M, Civin CI, Malech HL. Third-generation, self-inactivating gp91(phox) lentivector corrects the oxidase defect in NOD/SCID mouse-repopulating peripheral blood-mobilized CD34+ cells from patients with X-linked chronic granulomatous disease. Blood. 2002 Dec 15;100(13):4381-90.

Sachs MD, Schlechte H, Lenk VS, Brenner S, Schnorr D, Fleige B, Ditscherlein G, Loening SA. Genetic analysis of Tp53 from urine sediment as a tool for diagnosing recurrence and residual of bladder carcinoma. Eur Urol. 2000 Oct;38(4):426-33.

Schlechte HH, Sachs MD, Lenk SV, Brenner S, Rudolph BD, Loening SA. Progression in transitional cell carcinoma of the urinary bladder--analysis of Tp53 gene mutations by temperature gradients and sequence in tumor tissues and in cellular urine sediments. Cancer Detect Prev. 2000;24(1):24-32.


Prof. Martin Bornhäuser, Prof. Gerhard Ehninger Medizinische Klinik I, UKD, TU Dresden

Dr. Konstantinos Anastassiadis, CRTD, TU Dresden

Prof. Francis Stewart, Biotec, TU Dresden

Prof. Dr. Frank Buchholz, Medical Systems Biology, UKD, TU Dresden

Dr. Harry Malech, NIAID, NIH, USA

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