Funk Lab - NMR-based Metabolomics

NMR-based Metabolomics

Research Focus

Nuclear Magnetic Resonance (NMR) provides an exciting platform to investigate the quality of biofluids, as well their pre-analytical processes. With close ties to the Dresden Integrated Liquid Biobank (DILB), we are interested in investigating the role of NMR Spectroscopy in a clinical setting and as a quality control tool for biobanks.

As a part of the Quantitative Metabolomics Platform (QMP) we also interested in expanding our collaborative network for the measurement of metabolic profiles of clinical biosamples.

Specific expertise

Nuclear Magnetic Resonance
Magnetic Resonance Imaging
Metabolomics

Lab Members

Dr Funk, Alexander	Group leader	E-Mail
Jonas, Sophie	Technician (NMR)	E-Mail
Ciesielski, Piotr	Technician (SAMRI)	E-Mail

Professional Experience

Since 2024 Facility Manger for the Core Facility: Small Animal Magnetic Resonance Imaging (SAMRI), Dresden
Since 2021 Laboratory Manager for Dresden Integrated Liquid Biobank (DILB), Dresden
Since 2019 Facility Manager for Nuclear Magnetic Resonance, Institute for Clinical Chemistry & Laboratory Medicine, University Hospital Dresden, Dresden
2016 – 2019 Facility Manager for Nuclear Magnetic Resonance and small animal Magnetic Resonance Imaging Facility at the Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, Texas, USA
2015 - 2016 Postdoctoral Researcher, Group A. Dean Sherry, Advanced Imaging Research Center, UT Southwestern Medical Center, Dallas, Texas, USA
2011-2014 PhD Student, Thesis Topic: “Dissecting the Theories of Lanthanide Magnetic Resonance”, Group: David Parker, Department of Chemistry, Durham University, UK
2007-2011 Master of Chemistry (MChem), University of Manchester, UK

Selected Publications

Gebhardt M., Kunath C., Fröbel D., Funk A.M., Peitzsch M., Nölting S., Deutschbein T., Januszewicz A., Timmers H. JLM., Robledo M., Jahn A., Constantinescu G., Eisenhofer G., Pamporaki G., Richter S., Identification of succinate dehydrogenase gene variant carriers by blood biomarkers, J. Endoc. Soc, 2024, 8 (9), bvae142, https://doi.org/10.1210/jendso/bvae142
Von Renesse J, von Bechtolsheim F., Jonas S., Seifert L., Alvez T.C., Seifert A.M., Komorekt F., Guergana T., Menschikowski M., Bork U., Meisterfeld R., Distler M., Chavakis T., Weitz J., Funk A.M., Kahlert C., Mirtschink P., Tumour catabolism independent of malnutrition and inflammation in upper GI cancer patients revealed by longitudinal metabolomics, J. Cachexia Sarcopenia Muscle, 2023, 14 (1), 289-309. https://doi.org/10.1002/jcsm.13131
Jaschke, N. P.; Funk, A. M.; Jonas, S.; Riffel, R. M.; Sinha, A.; Wang, A.; Pählig, S.; Hofmann, M.; Altmann, H.; Von Bonin, S.; Koch, T.; Spieth, P.; Tausche, K.; Akgün, K.; Rauner, M.; Kronstein-Wiedemann, R.; Odendahl, M.; Tonn, T.; Göbel, A.; Hofbauer, L. C.; Rachner, T. D. Circulating Dickkopf1 Parallels Metabolic Adaptations and Predicts Disease Trajectories in Patients With COVID-19. J. Clin. Endoc2022, 107 (12), 3370–3377. https://doi.org/10.1210/clinem/dgac514.
Ratnakar, S. J.; Chirayil, S.; Funk, A. M.; Zhang, S.; Queiró, J. F.; Geraldes, C. F. G. C.; Kovacs, Z., Sherry, A. D. A Frequency-Selective PH-Responsive ParaCEST Agent. Angew. Chem. Int. Edit.2020, 59 (48), 21671–21676. https://doi.org/10.1002/anie.202008888.
Funk, A. M.; Wen, X.; Hever, T.; Maptue, N. R.; Khemtong, C.; Sherry, A. D.; Malloy, C. R. Effects of Deuteration on Transamination and Oxidation of Hyperpolarized C-13-Pyruvate in the Isolated Heart. J. Magn. Reson.2019, 301, 102–108. https://doi.org/10.1016/j.jmr.2019.03.003.
Courtney, K. D.; Bezwada, D.; Mashimo, T.; Pichumani, K.; Vemireddy, V.; Funk, A. M.; Wimberly, J.; McNeil, S. S.; Kapur, P.; Lotan, Y.; Margulis, V.; Cadeddu, J. A.; Pedrosa, I.; DeBerardinis, R. J.; Malloy, C. R.; Bachoo, R. M.; Maher, E. A. Isotope Tracing of Human Clear Cell Renal Cell Carcinomas Demonstrates Suppressed Glucose Oxidation In Vivo.Cell Metab.2018, 28 (5), 793-+. https://doi.org/10.1016/j.cmet.2018.07.020.
Funk, A. M.; Anderson, B. L.; Wen, X.; Hever, T.; Khemtong, C.; Kovacs, Z.; Sherry, A. D.; Malloy, C. R. The Rate of Lactate Production from Glucose in Hearts Is Not Altered by Per-Deuteration of Glucose.J. Magn. Reson.2017, 284, 86–93. https://doi.org/10.1016/j.jmr.2017.09.007.
Funk, A. M.; Jordan, V. C.; Sherry, A. D.; Ratnakar, S. J.; Kovacs, Z. Oxidative Conversion of a Europium(II)-Based T-1 Agent into a Europium(III)-Based ParaCEST Agent That Can Be Detected In Vivo by Magnetic Resonance Imaging.Angew. Chem.-Int. Edit.2016, 55 (16), 5024–5027. https://doi.org/10.1002/anie.201511649.
Funk, A. M.; Finney, K.-L. N. A.; Harvey, P.; Kenwright, A. M.; Neil, E. R.; Rogers, N. J.; Senanayake, P. K.; Parker, D. Critical Analysis of the Limitations of Bleaney’s Theory of Magnetic Anisotropy in Paramagnetic Lanthanide Coordination Complexes.Chem. Sci.2015, 6 (3), 1655–1662.

Collaborations

Name	Institute	Theme