Benutzerspezifische Werkzeuge

Neurochemistry

Platzhalter Bild

Prof. Dr. Graeme Eisenhofer

Group Leader


Research focus

Endocrine hypertension and adrenal tumors, this covering primary aldosteronism, pheochromocytoma, paraganglioma, hypercortisolism and related disorders of the hypothalamus-pituitary adrenal axis and sympathetic nervous system.  Research in these areas includes a focus on multidimensional diagnostics, artificial intelligence and metabologenomics to facilitate efficient diagnostic stratification for effective therapeutic interventions. Related bench level areas of research with a focus on the same disorders involve elucidation of tumorigenic pathways and development of targeted therapies using genetically manipulated cell line model systems.

Specific expertise

  • Forty years experience in patient-oriented research with a focus on analytical applications and diagnostics
  • Clinical research experience in autonomic pharmacology, disorders of the autonomic nervous system, cardiovascular disorders and endocrine tumors.
  • Broad hands-on experience in preclinical applications involving small to larger animals (mice, rats, rabbits, dogs, sheep) 
  • Expertise in applications of analytical methods for studies of the sympathetic nervous system and hypothalamus pituitary adrenal endocrine systems

Basic research and cell line models (Susan Richter, Nicole Bechmann)

  • pheochromocytoma, neuroblastoma and adrenocortical carcinoma cell line models
  • gene editing (transient and stable knockdowns, CRISPR/Cas9)
  • tumor cell biology (metabolism, migration/ invasion)
  • studies of small molecule inhibitors

Funding

Lab Members

Dr. Bechmann, NicolePostdoc
Conrad, CatleenTechnician
Dr. Constantinescu, GeorgianaStudy doctor
Prof. Dr. Eisenhofer, GraemeGroup leader
Fleischer, TinaTechnician
Helm, JanaPhD student
Kunath, CarolaStudy nurse
Langton, KatharinaStudy nurse
Li, MinghaoPhD student
Dr. Masjkur, JimmyStudy doctor
Dr. Pamporaki, ChristinaStudy doctor
Poser, IsabelTechnician
Dr. Richter, SusanPostdoc

Selected Publications

  •  Bechmann N, Moskopp ML, Ullrich M, Calsina B, Wallace PW, Richter S, Friedemann M, Langton K, Fliedner SM, Timmers HL, Nölting S, Beuschlein F, Fassnacht M, Preijbisz A, Pacak K, Ghayee HK, Bornstein SR, Dieterich P, Pietzsch J, Wielockx B, Robledo M, Qin N, Eisenhofer G. HIF2α supports pro-metastatic behavior in pseudohypoxic pheochromocytomas/paragangliomas. Endocr Relat Cancer 2020
  • Eisenhofer, G, Deutschbein, T, Constantinescu, G, Langton, K, Pamporaki, C, Calsina, B, Monteagudo, M, Peitzsch, M, Fliedner, S, Timmers, H, Bechmann, N, Fankhauser, M, Nolting, S, Beuschlein, F, Stell, A, Fassnacht, M, Prejbisz, A, Lenders, J W M and Robledo, M. Plasma metanephrines and prospective prediction of tumor location, size and mutation type in patients with pheochromocytoma and paraganglioma. Clin Chem Lab Med (2020)
  • Eisenhofer, G, Duran, C, Cannistraci, C V, Peitzsch, M, Williams, T A, Riester, A, Burrello, J, Buffolo, F, Prejbisz, A, Beuschlein, F, Januszewicz, A, Mulatero, P, Lenders, J W M and Reincke, M. Use of Steroid Profiling Combined With Machine Learning for Identification and Subtype Classification in Primary Aldosteronism. JAMA Netw Open 3: e2016209 (2020)
  • Jiang, J, Zhang, J, Pang, Y, Bechmann, N, Li, M, Monteagudo, M, Calsina, B, Gimenez-Roqueplo, A P, Nolting, S, Beuschlein, F, Fassnacht, M, Deutschbein, T, Timmers, H, Akerstrom, T, Crona, J, Quinkler, M, Fliedner, S M J, Liu, Y, Guo, J, Li, X, Guo, W, Hou, Y, Wang, C, Zhang, L, Xiao, Q, Liu, L, Gao, X, Burnichon, N, Robledo, M and Eisenhofer, G. Sino-European Differences in the Genetic Landscape and Clinical Presentation of Pheochromocytoma and Paraganglioma. J Clin Endocrinol Metab 105(2020)
  • Peitzsch, M, Butch, E R, Lovorn, E, Mangelis, A, Furman, W L, Santana, V M, Hero, B, Berthold, F, Shulkin, B L, Huebner, A and Eisenhofer, G. Biochemical testing for neuroblastoma using plasma free 3-O-methyldopa, 3-methoxytyramine, and normetanephrine. Pediatr Blood Cancer 67: e28081 (2020)
  • Wallace, P W, Conrad, C, Bruckmann, S, Pang, Y, Caleiras, E, Murakami, M, Korpershoek, E, Zhuang, Z, Rapizzi, E, Kroiss, M, Gudziol, V, H, J L M T, Mannelli, M, Pietzsch, J, Beuschlein, F, Pacak, K, Robledo, M, Klink, B, Peitzsch, M, Gill, A J, Tischler, A S, de Krijger, R R, Papathomas, T, Aust, D, Eisenhofer, G and Richter, S. Metabolomics, machine learning and immunohistochemistry to predict succinate dehydrogenase mutational status in phaeochromocytomas and paragangliomas. J Pathol, 251:378-387 (2020)
  • Bechmann, N, Poser, I, Seifert, V, Greunke, C, Ullrich, M, Qin, N, Walch, A, Peitzsch, M, Robledo, M, Pacak, K, Pietzsch, J, Richter, S and Eisenhofer, G. Impact of Extrinsic and Intrinsic Hypoxia on Catecholamine Biosynthesis in Absence or Presence of Hif2alpha in Pheochromocytoma Cells. Cancers (Basel)11(2019)
  • Fankhauser, M, Bechmann, N, Lauseker, M, Goncalves, J, Favier, J, Klink, B, William, D, Gieldon, L, Maurer, J, Spottl, G, Rank, P, Knosel, T, Orth, M, Ziegler, C G, Aristizabal Prada, E T, Rubinstein, G, Fassnacht, M, Spitzweg, C, Grossman, A B, Pacak, K, Beuschlein, F, Bornstein, S R, Eisenhofer, G, Auernhammer, C J, Reincke, M and Nolting, S. Synergistic Highly Potent Targeted Drug Combinations in Different Pheochromocytoma Models Including Human Tumor Cultures. Endocrinology160: 2600-2617 (2019)
  • Richter, S, Gieldon, L, Pang, Y, Peitzsch, M, Huynh, T, Leton, R, Viana, B, Ercolino, T, Mangelis, A, Rapizzi, E, Menschikowski, M, Aust, D, Kroiss, M, Beuschlein, F, Gudziol, V, Timmers, H J, Lenders, J, Mannelli, M, Cascon, A, Pacak, K, Robledo, M, Eisenhofer, G and Klink, B. Metabolome-guided genomics to identify pathogenic variants in isocitrate dehydrogenase, fumarate hydratase, and succinate dehydrogenase genes in pheochromocytoma and paraganglioma. Genet Med21: 705-717 (2019)
  • Bechmann, N, Ehrlich, H, Eisenhofer, G, Ehrlich, A, Meschke, S, Ziegler, C G and Bornstein, S R. Anti-Tumorigenic and Anti-Metastatic Activity of the Sponge-Derived Marine Drugs Aeroplysinin-1 and Isofistularin-3 against Pheochromocytoma In Vitro. Mar Drugs16(2018)
  • Eisenhofer, G, Prejbisz, A, Peitzsch, M, Pamporaki, C, Masjkur, J, Rogowski-Lehmann, N, Langton, K, Tsourdi, E, Peczkowska, M, Fliedner, S, Deutschbein, T, Megerle, F, Timmers, H, Sinnott, R, Beuschlein, F, Fassnacht, M, Januszewicz, A and Lenders, J W M. Biochemical Diagnosis of Chromaffin Cell Tumors in Patients at High and Low Risk of Disease: Plasma versus Urinary Free or Deconjugated O-Methylated Catecholamine Metabolites. Clin Chem64: 1646-1656 (2018)
  • Richter, S, D'Antongiovanni, V, Martinelli, S, Bechmann, N, Riverso, M, Poitz, D M, Pacak, K, Eisenhofer, G, Mannelli, M and Rapizzi, E. Primary fibroblast co-culture stimulates growth and metabolism in Sdhb-impaired mouse pheochromocytoma MTT cells. Cell Tissue Res374: 473-485 (2018)
  • Eisenhofer, G, Dekkers, T, Peitzsch, M, Dietz, A S, Bidlingmaier, M, Treitl, M, Williams, T A, Bornstein, S R, Haase, M, Rump, L C, Willenberg, H S, Beuschlein, F, Deinum, J, Lenders, J W and Reincke, M. Mass Spectrometry-Based Adrenal and Peripheral Venous Steroid Profiling for Subtyping Primary Aldosteronism. Clin Chem62: 514-24 (2016)
  • Qin, N, de Cubas, A A, Garcia-Martin, R, Richter, S, Peitzsch, M, Menschikowski, M, Lenders, J W, Timmers, H J, Mannelli, M, Opocher, G, Economopoulou, M, Siegert, G, Chavakis, T, Pacak, K, Robledo, M and Eisenhofer, G. Opposing effects of HIF1alpha and HIF2alpha on chromaffin cell phenotypic features and tumor cell proliferation: Insights from MYC-associated factor X. Int J Cancer135: 2054-64 (2014)
  • Eisenhofer, G, Lenders, J W, Timmers, H, Mannelli, M, Grebe, S K, Hofbauer, L C, Bornstein, S R, Tiebel, O, Adams, K, Bratslavsky, G, Linehan, W M and Pacak, K. Measurements of plasma methoxytyramine, normetanephrine, and metanephrine as discriminators of different hereditary forms of pheochromocytoma. Clin Chem57: 411-20 (2011)
  • Eisenhofer, G, Kopin, I J and Goldstein, D S. Catecholamine metabolism: a contemporary view with implications for physiology and medicine. Pharmacol Rev56: 331-49 (2004)
  • Lenders, J W, Pacak, K, Walther, M M, Linehan, W M, Mannelli, M, Friberg, P, Keiser, H R, Goldstein, D S and Eisenhofer, G. Biochemical diagnosis of pheochromocytoma: which test is best? JAMA287: 1427-34 (2002)
  • Eisenhofer, G, Lenders, J W, Linehan, W M, Walther, M M, Goldstein, D S and Keiser, H R. Plasma normetanephrine and metanephrine for detecting pheochromocytoma in von Hippel-Lindau disease and multiple endocrine neoplasia type 2. N Engl J Med340: 1872-9 (1999)
  • Eisenhofer, G, Friberg, P, Rundqvist, B, Quyyumi, A A, Lambert, G, Kaye, D M, Kopin, I J, Goldstein, D S and Esler, M D. Cardiac sympathetic nerve function in congestive heart failure. Circulation93: 1667-76 (1996)
  • Eisenhofer, G and Johnson, R H. Effect of ethanol ingestion on plasma vasopressin and water balance in humans. Am J Physiol242: R522-7 (1982)