Benutzerspezifische Werkzeuge

Experimental Mass Spectrometry Unit (EMS)

Platzhalter Bild

Dr. Mirko Peitzsch

Group Leader

Focus

Changes in metabolism are considered the functional readout of anything happening to a biological system. Therefore, metabolomics analyses of small molecules in body fluids, cells, tissues and tissue biopsies provide comprehensive insights into metabolism. In the Experimental Mass Spectrometry unit (EMS) different liquid chromatography tandem mass spectrometry (LC-MS/MS) setups are available for sensitive and specific, quantitative and qualitative analysis of small molecule metabolites. Depending the research question and the experimental design different approaches for targeted quantitative metabolite profiling or screening and identification of metabolites using an untargeted metabolomics setting exist.

Specific expertise

  • LC-MS/MS based analysis of small molecules in biological matrices
  • Development of LC-MS/MS based methods for metabolite profiling
  • Planning, analysis and interpretation of data

EMS user guidelines are available on request.

Links

Dresden Campus MS community:

Funding

 

Lab Members

Fröbel, Dennis Technician
Kaden, DeniseTechnician
Langner, MatthiasPhD-student
Dr. Peitzsch, MirkoGroup leader

Selected Publications

  • Bechmann N, Watts D, Steenblock C, Wallace PW, Schürmann A, Bornstein SR, Wielockx B, Eisenhofer G, Peitzsch M. Adrenal hormone interactions and metabolism: A single sample multi-omics approach. Horm Metab Res 2021; in press
  • Peitzsch M, Novos T, Kaden D, Kurlbaum M, van Herwaarden AE, Müller D, Adaway J, Grouzmann E, McWhinney B, Hoad K, Woolard G, Kema I, Boot C, Fassnacht M, Sweep F, Loh TP, Horvath AR, Eisenhofer G. Harmonization of LC-MS/MS measurements of plasma free normetanephrine, metanephrine and 3-methoxytyramine. Clin Chem 2021; in press
  • Peitzsch M, Butch ER, Lovorn E, Mangelis A, Furman WL, Santana VM, Hero B, Berthold F, Shulkin BL, Huebner A, Eisenhofer G. Biochemical testing for neuroblastoma using plasma free 3-O-methyldopa, 3-methoxytyramine, and normetanephrine. Pediatr Blood Cancer 2020; 67(2):e28081
  • Peitzsch M, Mangelis A, Eisenhofer G, Huebner A. Age-specific pediatric reference intervals for plasma free normetanephrine, metanephrine, 3-methoxytyramine and 3-O-methyldopa: particular importance for early infancy. Clin Chim Acta 2019; 494:100-5
  • Masjkur J, Gruber M, Peitzsch M, Kaden D, Di Dalmazi G, Bidlingmaier M, Zopp S, Langton K, Fazel J, Beuschlein F, Bornstein SR, Reincke M, Eisenhofer G. Plasma steroid profiles in subclinical compared with overt adrenal Cushing Syndrome. J Clin Endocrinol Metab 2019;104(10):4331-4340
  • Eisenhofer G, Masjkur J, Peitzsch M, Di Dalmazi G, Bidlingmaier M, Gruber M, Fazel J, Osswald A, Beuschlein F, Reincke M. Plasma steroid metabolome profiling for diagnosis and subtyping patients with Cushing Syndrome. Clin Chem 2018;64(3):586-596
  • Eisenhofer G, Peitzsch M, McWhinney BC. Impact of LC-MS/MS on the laboratory diagnosis of catecholamine-producing tumors. TRAC-Trends in Analytical Chemistry 2016; 84:106-115
  • Eisenhofer G, Dekkers T, Peitzsch M, Dietz AS, Bidlingmaier M, Treitl M, Williams TA, Bornstein SR, Haase M, Rump LC, Willenberg HS, Beuschlein F, Deinum J, Lenders JWM, Reincke M. Mass spectrometry-based adrenal and peripheral venous steroid profiling for subtyping of primary aldosteronism. Clin Chem 2016;62(3):514-524
  • Williams TA, Peitzsch M, Dietz AS, Dekkers T, Bidlingmaier M, Riester A, Treitl M, Rhayem Y, Beuschlein F, Lenders JW, Deinum J, Eisenhofer G, Reincke M. Genotype-specific steroid profiles associated with aldosterone-producing adenomas. Hypertension 2016; 67(1): 139-45
  • Peitzsch M, Adaway JE, Eisenhofer G. Interference from 3-O-methyldopa with ultra-high performance LC-MS/MS measurements of plasma metanephrines: Chromatographic separation remains important. Clin Chem 2015; 61(7): 993-6
  • Peitzsch M, Dekkers T, Haase M, Sweep FC, Quack I, Antoch G, Siegert G, Lenders JW, Deinum J, Willenberg HS, Eisenhofer G. An LC-MS/MS method for steroid profiling during adrenal venous sampling for investigation of primary aldosteronism. J Steroi Biochem Mol Biol 2015; 145:75-84
  • Richter S, Peitzsch M, Rapizzi E, Lenders JW, Qin N, de Cubas AA, Schiavi F, Rao JU, Beuschlein F, Quinkler M, Timmers HJ, Opocher G, Mannelli M, Robledo M, Eisenhofer G. Krebs cycle metabolite profiling for identification and stratification of pheochromocytomas/paragangliomas due to succinate dehydrogenase deficiency. J Clin Endocrinol Metab. 2014; 99(10):3903-11
  • Weismann D, Peitzsch M, Raida A, Prejbisz A, Gosk M, Riester A, Willenberg HS, Klemm R, Manz G, Deutschbein T, Kroiss M, Darr R, Bidlingmaier M, Januszewicz A, Eisenhofer G, Fassnacht M. Measurements of plasma metanephrines by immunoassay vs liquid chromatography with tandem mass spectrometry for diagnosis of pheochromocytoma. Eur J Endocrinol. 2014; 172(3):251-60
  • Juratli TA*, Peitzsch M*, Geiger K, Schackert G, Eisenhofer G, Krex, Dietmar. Accumulation of 2-hydroxyglutarate is not a biomarker for malignant progression in IDH-mutated low-grade gliomas. Neuro Oncol 2013; 15(6):682-90
  • Peitzsch M, Prejbisz A, Kroiss M, Beuschlein F, Arlt W, Januszewicz A, Siegert G, Eisenhofer G. Analysis of plasma 3-methoxytyramine, normetanephrine and metanephrine by ultra performance liquid chromatography - tandem mass spectrometry: utility for diagnosis of dopamine-producing metastatic phaeochromocytoma. Ann Clin Biochem 2013; 50(2):147-55
  • Peitzsch M, Pelzel D, Glockner S, Prejbisz A, Fassnacht M, Beuschlein F, Januszewicz A, Siegert G, Eisenhofer G. Simultaneous liquid chromatography tandem mass spectrometric determination of urinary free metanephrines and catecholamines, with comparisons of free and deconjugated metabolites, Clin Chim Acta 2013; 418:50-8