Benutzerspezifische Werkzeuge

HPLC-ECD/UV Unit

KFO252_long

HPLC-ECD/UV Unit

Head: Peter Lattke, PhD
Staff: Daniela Grossman (Technical assistant)

The HPLC-ECD/UV unit represents the first established unit and the basis under which the Division was first founded. The unit hosts eight HPLC systems, most of which are equipped with electrochemical detectors (ECD). Under the supervision of Dr. Lattke, the unit is devoted principally to routine laboratory analytics, with assays covering urinary metanephrines, catecholamines, homovanillic acid, vanillylmandelic acid 5-hydroxyindloeacetic acid, as well as several other methods utilizing UV detection for measurements of drugs and vitamins. Under the additional co-supervision by Dr. Nan Qin, the unit’s Chief analyst who also heads the Cell Biology unit, three of the HPLC systems are dedicated to supporting the Division’s research into catecholamine systems. This includes established methods for measuring catechols (norepinephrine, epinephrine, dopamine, 3,4-dihydroxyphenylglycol, 3,4-dihydroxyphenylalanine and 3,4-dihydroxyphenylacetic acid) in plasma, urine, CSF and tissue samples. Assays to quantify activities of enzymes involved in catecholamine synthesis and metabolism (e.g., tyrosine hydroxylase, catechol-O-methyltransferase) are also available to support the research of the Division and collaborating investigators.

Selected Publications

  1. Lattke, P., M. Peitzsch, R. Därr, G. Siegert and G. Eisenhofer. Labordiagnostik Katecholamin-produzierender Tumoren. LaboratoriumsMedizin 37, 187-97, 2013.
  2. Eisenhofer G, Lattke P, Herberg M, Siegert G, Qin N, Därr R, Hoyer J, Villringer A, Prejbisz A, Januszewicz A, Remaley A, Martucci V, Pacak K, Ross HA, Sweep FC, Lenders JW. Reference intervals for plasma free metanephrines with an age adjustment for normetanephrine for optimized laboratory testing of phaeochromocytoma Ann Clin Biochem. 2013,50: 62-9.
  3. Eisenhofer G, Pacak K, Huynh TT, Qin N, Bratslavsky G, Linehan WM, Mannelli M, Friberg P, Grebe SK, Timmers HJ, Bornstein SR, Lenders JW. Catecholamine metabolomic and secretory phenotypes in phaeochromocytoma. Endocr Relat Cancer. 2010 Dec 21;18:97-111