Spectrum of techniques/methods

DNA/RNA/microRNA isolation from cell and tissue samples
Quantitative PCR: quantitation of gene expression, determination of gene copy number, detection of SNPs
Transcriptome-wide gene expression analysis using oligonucleotide microarrays (Affymetrix technology)
Protein analysis (Western blot, ELISA, immunohistochemistry, immunofluorescence)
Culture of human tumor cell lines and non-malignant cells
3D-culture of human tumor cells (multicellular tumor spheroids)
Stable and transient transfection of tumor cells
Knock-down of gene expression by antisense oligodeoxynucleotides and siRNAs
Modulation of microRNA Expression, reporter gene assays
Assays for the characterization of cell growth (proliferation, apoptosis, cell cycle, cell colony formation, wound healing, migration and invasion)
Flow cytometry and immunomagnetic cell separation
Functionalization as well as analysis of cytotoxicity and biocompatibility of carbon nanomaterials
Development of human bladder cancer xenograft models in mice for the in vivo testing of anticancer agents
Tissue storage (cryo and paraffin), histological and immunohistochemical stainings
Generation of tissue microarrays
Cloning and sequencing
Fluorescence microscopy