Benutzerspezifische Werkzeuge

Spectrum of techniques/methods

  • DNA/RNA/microRNA isolation from cell and tissue samples

  • Quantitative PCR: quantitation of gene expression, determination of gene copy number, detection of SNPs
  • Transcriptome-wide gene expression analysis using oligonucleotide microarrays (Affymetrix technology)
  • Protein analysis (Western blot, ELISA, immunohistochemistry, immunofluorescence)
  • Culture of human tumor cell lines and non-malignant cells
  • 3D-culture of human tumor cells (multicellular tumor spheroids)
  • Stable and transient transfection of tumor cells
  • Knock-down of gene expression by antisense oligodeoxynucleotides and siRNAs
  • Modulation of microRNA Expression, reporter gene assays
  • Assays for the characterization of cell growth (proliferation, apoptosis, cell cycle, cell colony formation, wound healing, migration and invasion)
  • Flow cytometry and immunomagnetic cell separation
  • Functionalization as well as analysis of cytotoxicity and biocompatibility of carbon nanomaterials
  • Development of human bladder cancer xenograft models in mice for the in vivo testing of anticancer agents
  • Tissue storage (cryo and paraffin), histological and immunohistochemical stainings
  • Generation of tissue microarrays
  • Cloning and sequencing
  • Fluorescence microscopy