
- Info
Spectrum of techniques/methods
- DNA/RNA/microRNA isolation from cell and tissue samples

- Quantitative PCR: quantitation of gene expression, determination of gene copy number, detection of SNPs
- Transcriptome-wide gene expression analysis using oligonucleotide microarrays (Affymetrix technology)
- Protein analysis (Western blot, ELISA, immunohistochemistry, immunofluorescence)

- Culture of human tumor cell lines and non-malignant cells
- 3D-culture of human tumor cells (multicellular tumor spheroids)
- Stable and transient transfection of tumor cells
- Knock-down of gene expression by antisense oligodeoxynucleotides and siRNAs
- Modulation of microRNA Expression, reporter gene assays
- Assays for the characterization of cell growth (proliferation, apoptosis, cell cycle, cell colony formation, wound healing, migration and invasion)

- Flow cytometry and immunomagnetic cell separation
- Functionalization as well as analysis of cytotoxicity and biocompatibility of carbon nanomaterials
- Development of human bladder cancer xenograft models in mice for the in vivo testing of anticancer agents
- Tissue storage (cryo and paraffin), histological and immunohistochemical stainings

- Generation of tissue microarrays
- Cloning and sequencing
- Fluorescence microscopy