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Experimentelle Hämatologie
Leiter
OA PD Dr. med. Sebastian Brenner (Foto)
Wissenschaftliche Mitarbeiter
Dr. rer. nat. Sebastian Thieme
Dr. rer. nat. Magdalena Laugsch
Dr. rer. nat. Cornelia Richter
Dr. med. Tobias Gayrfas
Joanna Bandola MSc
David Brandt
Cand. med. Paula Jobst
cand. med. Malte Lehmann
Katrin Navratiel (MTA)
Jenny Marzahn (MTA)
Betreuung wissenschaftlicher Mitarbeiter in Kooperation
Dr. med. Martin Wermke (Kooperation mit Prof. Buchholz und Prof. Bornhäuser)
Aylin Camgoz, MSc (Kooperation mit Prof. Buchholz und Prof. Bornhäuser)
Dipl. Biol. Sabine Stopp (Kooperation mit Prof. Bornhäuser)
Dipl. Biol. Regina Duryagina (Kooperation mit Prof. Bornhäuser und Prof. Carsten Werner)
Forschungsprojekte
- Erstellung und Korrektur Patienten-spezifischer iPS Zellen zur Therapie der Septischen Granulomatose (BMBF-Projekt in Kooperation mit Dr. Konstantinos Anastassiadis und Prof. Francis Stewart; Dr. Laugsch)
- Rolle der H3K27 Demethylase UTX bei der Blutstammzell-Migration (SFB 655, B6; Dr. Thieme)
- Charakterisierung neuer Mechanismen der Migration hämatopoetischer Stammzellen(MeDDrive 2011/2012, Dr. Gyarfas)
- Rolle der plasmazytoiden dendritischen Zellen bei Asthma bronchiale (DFG Projekt, Joanna Bandola, MSc)
- Die Rückkopplung der IL-12p70 Antwort durch Ncf1 (p47phox) und seine Bedeutung in der Tumorprävention (DFG, Dr. Richter)
- shRNA Screen zur Identifikation neuer, an der Blutstammzell-Migration und Zelladhäsion beteiligter Gene
- Rolle von MCAM auf mesenchymalen Stammzellen (Sabine Stopp)
- Etablierung eines deutschlandweiten pädiatrischen Reanimations- und Hypothermieregisters
ausgewählte Publikationen
Jacobi A, Thieme S, Lehmann R, Ugarte F, Malech HL, Koch S, Thiede C, Müller K, Bornhäuser M, Ryser M, Brenner S. Impact of CXCR4 inhibition on FLT3-ITD positive human AML blasts. Exp Hematol. 2010;38:180-190.
Ugarte F, Ryser M, Thieme S, Fierro FA, Navratiel K, Bornhäuser M, Brenner S. Notch signaling enhances osteogenic differentiation while inhibiting adipogenesis in primary human bone marrow stromal cells. Exp Hematol. 2009 Jul;37(7):867-875.
Ryser MF, Thieme S, Lehmann R, Bornhäuser M, Brenner S. Serum albumin strongly influences SDF-1 dependent migration. Int J Hematol. 2009 Apr;89(3):269-75.
Fierro FA, Brenner S, Oelschlaegel U, Jacobi A, Knoth H, Ehninger G, Illmer T, Bornhäuser M: Combining SDF-1/CXCR4 antagonism and chemotherapy in relapsed acute myeloid leukemia. Leukemia. 2009 Feb;23(2):393-6.
Moreno-Carranza B, Gentsch M, Stein S, Schambach A, Santilli G, Rudolf E, Ryser MF, Haria S, Thrasher AJ, Baum C, Brenner S, Grez M. Transgene optimization significantly improves SIN vector titers, gp91phox expression and reconstitution of superoxide production in X-CGD cells. Gene Ther. 2009 Jan16(1):111-8.
Ryser MF, Ugarte F, Gentsch M, Lehmann R, Bornhäuser M, Brenner S. S1P1 overexpression stimulates S1P dependent chemotaxis of human hematopoietic stem cells but strongly inhibits SDF-1/CXCR4 dependent migration and in vivo homing. Mol Immunol. 2008 Nov;46,166-71.
Ryser MF, Ugarte F, Thieme S, Gentsch M, Bornhäuser M, Roesen-Wolff A, Brenner S. mRNA transfection of a CXCR4-GFP fusion protein - generated by a simple three step PCR protocol - results in efficient migration of primary mesenchymal stem cells. Tissue Eng Part C Methods. 2008 Sep;14,179-84.
Gene Therapy for Chronic Granulomatous Disease. Ryser MF, Roesler J, Gentsch M, Brenner S. Expert Opinion on Biological Therapy. 2007 Dec;7(12): 1799-1809.
The late dividing population of gamma-retroviral vector transduced human mobilized peripheral blood progenitor cells contributes most to gene-marked cell engraftment in nonobese diabetic/severe combined immunodeficient mice. Brenner S, Ryser MF, Whiting-Theobald NL, Gentsch M, Linton GF, Malech HL. Stem Cells. 2007 Jul;25(7):1807-13.
Brenner S, Ryser MF, Choi U, Whiting-Theobald N, Kuhlisch E, Linton G, Kang E, Lehmann R, Rosen-Wolff A, Rudikoff AG, Farese AM, MacVittie TJ, Roesler J, Horwitz ME, Malech HL. Polyclonal long-term MFGS-gp91phox marking in rhesus macaques after non-myeloablative transplantation with transduced autologous peripheral blood progenitor cells. Mol Ther. 2006 Aug;14(2):202-11.
Ueda T, Brenner S, Malech HL, Langemeijer SM, Perl S, Kirby M, Phang OA, Krouse AE, Donahue RE, Kang EM, Tisdale JF. Cloning and functional analysis of the rhesus macaque ABCG2 gene. Forced expression confers an SP phenotype among hematopoietic stem cell progeny in vivo. J Biol Chem. 2005 Jan 14;280(2):991-8.
Brenner S, Whiting-Theobald N, Kawai T, Linton GF, Rudikoff AG, Choi U, Ryser MF, Murphy PM, Sechler JM, Malech HL. CXCR4-transgene expression significantly improves marrow engraftment of cultured hematopoietic stem cells. Stem Cells. 2004;22(7):1128-33.
Malech HL, Choi U, Brenner S. Progress toward effective gene therapy for chronic granulomatous disease. Jpn J Infect Dis. 2004 Oct;57(5):S27-8.
Brenner S, Whiting-Theobald NL, Linton GF, Holmes KL, Anderson-Cohen M, Kelly PF, Vanin EF, Pilon AM, Bodine DM, Horwitz ME, Malech HL. Concentrated RD114-pseudotyped MFGS-gp91phox vector achieves high levels of functional correction of the chronic granulomatous disease oxidase defect in NOD/SCID/beta -microglobulin-/- repopulating mobilized human peripheral blood CD34+ cells. Blood. 2003 Oct 15;102(8):2789-97.
Brenner S, Malech HL. Current developments in the design of onco-retrovirus and lentivirus vector systems for hematopoietic cell gene therapy. Biochim Biophys Acta. 2003 Apr 7;1640(1):1-24.
Brenner S, Roesler J, Bukovsky AA, Whiting-Theobald N, Dull T, Kelly M, Civin CI, Malech HL. Third-generation, self-inactivating gp91(phox) lentivector corrects the oxidase defect in NOD/SCID mouse-repopulating peripheral blood-mobilized CD34+ cells from patients with X-linked chronic granulomatous disease. Blood. 2002 Dec 15;100(13):4381-90.
Kooperationen:
Prof. Martin Bornhäuser, Prof. Gerhard Ehninger Medizinische Klinik I, UKD, TU Dresden
Dr. Konstantinos Anastassiadis, CRTD, TU Dresden
Prof. Francis Stewart, Biotec, TU Dresden
Prof. Dr. Frank Buchholz, Medical Systems Biology, UKD, TU Dresden
Dr. Harry Malech, NIAID, NIH, USA
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